Human IL-17RA Recombinant Rabbit Monoclonal Antibody [PSH24-65]
cat.: HA724461
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-17RA aa 33-320 (HA210581). |
| Positive control: | Recombinant Human IL-17RA protein (HA210581). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-66] to Human IL-17RA (Detecor) (HA724462) and Recombinant Human IL-17RA protein (HA210581) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: Q96F46 Human |
| Alternative names: | CANDF5 CD217 CD217 antigen CDw217 CTLA8 HIL 17R hIL17R I17RA_HUMAN IL 17 receptor A IL 17 receptor IL 17RA IL-17 receptor A IL-17RA IL17 IL17A receptor IL17R IL17RA IMD51 Interleukin 17 receptor A Interleukin-17 receptor A MGC10262 |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-17RA matched pair antibodies Capture: HA724461, Human IL-17RA Rabbit mAb [PSH24-65] Detector: HA724462, Human IL-17RA Rabbit mAb [PSH24-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724461) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-17RA protein (HA210581) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (HA724462, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL-17RA in THP-1 and Jurkat cell extract samples based on a 1,000 µg/mL extract load. Capture: HA724461, Human IL-17RA Rabbit mAb [PSH24-65] Detector: HA724462, Human IL-17RA Rabbit mAb [PSH24-66] The concentrations of IL-17RA were measured in duplicates, interpolated from the IL-17RA standard curve and corrected for sample dilution. Undiluted samples are THP-1 cell extract 50% and Jurkat cell extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17RA concentration was determined to be 2,342 pg/mL in THP-1 cell extract and 655 pg/mL in Jurkat cell extract. |
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Fig3:
Interpolated concentrations of spiked IL-17RA in human cell culture media samples. Capture: HA724461, Human IL-17RA Rabbit mAb [PSH24-65] Detector: HA724462, Human IL-17RA Rabbit mAb [PSH24-66] The concentrations of IL-17RA were measured in duplicates, interpolated from the IL-17RA standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.