Human Pro-Collagen alpha-2 C-Terminal Propeptide Recombinant Rabbit Monoclonal Antibody [PSH24-68]
cat.: HA724464
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Pro-Collagen alpha-2 C-Terminal Propeptide aa 1,121-1,366 (HA211385). |
| Positive control: | Recombinant Human Pro-Collagen alpha-2 C-Terminal Propeptide protein (HA211385). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-67] to Human Pro-Collagen alpha-2 C-Terminal Propeptide (Capture) (HA724463) and Recombinant Human Pro-Collagen alpha-2 C-Terminal Propeptide protein (HA211385) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: P08123 Human |
| Alternative names: | Alpha 2 collagen type I Alpha 2 type I collagen Alpha 2 type I procollagen Alpha 2(I) collagen Alpha 2(I) procollagen Alpha-2 type I collagen CO1A2_HUMAN COL1A2 Collagen alpha 2(I) chain Collagen alpha-2(I) chain Collagen I alpha 2 polypeptide Collagen of skin tendon and bone alpha 2 chain Collagen type I alpha 2 OI4 Osteogenesis imperfecta type IV Type I procollagen |
Images
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Fig1:
Sandwich ELISA analysis of Human Pro-Collagen alpha-2 C-Terminal Propeptide matched pair antibodies Capture: HA724463, Human Pro-Collagen alpha-2 C-Terminal Propeptide Rabbit mAb [PSH24-67] Detector: HA724464, Human Pro-Collagen alpha-2 C-Terminal Propeptide Rabbit mAb [PSH24-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724463) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Pro-Collagen alpha-2 C-Terminal Propeptide protein (HA211385) starting from 2,000 pg/mL to 0 pg/mL and detect antibody (HA724464, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Pro-Collagen alpha-2 C-Terminal Propeptide in human samples. Capture: HA724463, Human Pro-Collagen alpha-2 C-Terminal Propeptide Rabbit mAb [PSH24-67] Detector: HA724464, Human Pro-Collagen alpha-2 C-Terminal Propeptide Rabbit mAb [PSH24-68] Interpolated concentration of native Human Pro-Collagen alpha-2 C-Terminal Propeptide was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Human Pro-Collagen alpha-2 C-Terminal Propeptide concentration was determined to be 119,071 pg/mL in cell culture supernatant and 781 pg/mL in human serum. There was no detectable signal in HeLa cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked Pro-Collagen alpha-2 C-Terminal Propeptide in cell culture media samples. Capture: HA724463, Human Pro-Collagen alpha-2 C-Terminal Propeptide Rabbit mAb [PSH24-67] Detector: HA724464, Human Pro-Collagen alpha-2 C-Terminal Propeptide Rabbit mAb [PSH24-68] The concentrations of Human Pro-Collagen alpha-2 C-Terminal Propeptide were measured in duplicates, interpolated from the Human Pro-Collagen alpha-2 C-Terminal Propeptide standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.