Endomucin Recombinant Rabbit Monoclonal Antibody [PSH24-71]
cat.: HA724467
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH24-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Endomucin aa 1-190. |
| Positive control: | Mouse kidney tissue lysate, Mouse thyroid tissue lysate, Mouse lung tissue lysate, Mouse heart tissue lysate, Mouse ovary tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:5,000 1:2,000 1:200 |
| Uniprot #: | SwissProt: Q9R0H2 Mouse |
| Alternative names: | EMCN EMCN2 Endomucin 2 Endomucin Endomucin-2 Endothelial sialomucin Gastric cancer antigen Ga34 MUC-14 MUC14 MUCEN_HUMAN Mucin 14 Mucin-14 |
Images
|
Fig1:
Western blot analysis of Endomucin on different lysates with Rabbit anti-Endomucin antibody (HA724467) at 1/5,000 dilution.
Lane 1: Mouse kidney tissue lysate Lane 2: Mouse thyroid tissue lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Mouse ovary tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Low expression : Mouse brain (PMID: 12000719). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724467, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 70-100 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724467, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724467, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724467, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.