HRP Conjugated Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43]
cat.: HA724470H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey, Pig |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JM42-43 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Vinculin aa 1,000-1,060. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Pig brain tissue lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Mouse colon tissue lysate, Rat lung tissue lysate, Rat spleen tissue lysate, Rat colon tissue lysate. |
| Subcellular location: | Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane. |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P18206 Human | Q64727 Mouse | P85972 Rat |
| Alternative names: | CMD1W CMH15 Epididymis luminal protein 114 HEL114 Metavinculin MV MVCL OTTHUMP00000019861 OTTHUMP00000019862 VCL VINC VINC_HUMAN Vinculin |
Images
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Fig1:
Western blot analysis of Vinculin on different lysates with Rabbit anti-Vinculin antibody (HA724470H) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 5: C6 (Rat glioma cell) cell lysate Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate Lane 7: Pig brain tissue lysate Lysates/proteins at 5 µg/Lane. Predicted band size: 124 kDa Observed band size: 124 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724470H) at 1/2,000 dilution was used in K1803 at room temperature for 1 hour. |
|
Fig2:
Western blot analysis of Vinculin on different lysates with Rabbit anti-Vinculin antibody (HA724470H) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate (10 µg/Lane) Lane 2: Mouse spleen tissue lysate (10 µg/Lane) Lane 3: Mouse colon tissue lysate (10 µg/Lane) Lane 4: Rat lung tissue lysate (10 µg/Lane) Lane 5: Rat spleen tissue lysate (10 µg/Lane) Lane 6: Rat colon tissue lysate (10 µg/Lane) Predicted band size: 124 kDa Observed band size: 124, ~145 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724470H) at 1/2,000 dilution was used in K1803 at room temperature for 1 hour. |
|
Fig3:
Western blot analysis of Vinculin on different lysates with Rabbit anti-Vinculin antibody (HA724470H) at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate (10 µg/Lane) Lane 2: Mouse spleen tissue lysate (10 µg/Lane) Lane 3: Mouse colon tissue lysate (10 µg/Lane) Lane 4: Rat lung tissue lysate (10 µg/Lane) Lane 5: Rat spleen tissue lysate (10 µg/Lane) Lane 6: Rat colon tissue lysate (10 µg/Lane) Predicted band size: 124 kDa Observed band size: 124, ~145 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724470H) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 1 hour. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.