SMCR7L / MID51 Recombinant Rabbit Monoclonal Antibody [PSH24-77]
cat.: HA724474
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey, Pig |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SMCR7L / MID51 aa 121-360. |
| Positive control: | LNCaP (Human prostate cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, K-562 (Human chronic myelogenous leukemia ) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Vero (African green monkey normal kidney cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, 4T1 (Mouse breast cancer cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse testis tissue lysate, Mouse heart tissue lysate, Rat testis tissue lysate, Pig brain tissue lysate, Pig cerebellum tissue lysate. |
| Subcellular location: | Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9NQG6 Human | Q8BGV8 Mouse | Q5XIS8 Rat |
| Alternative names: | dJ1104E15.3 HSU79252 Hypothetical protein LOC54471 MID51 MIEF1 mitochondrial dynamic protein MID51 mitochondrial dynamic protein of 51 kDa mitochondrial dynamics protein MID51 mitochondrial dynamics protein of 51 kDa mitochondrial elongation factor 1 SMC7L_HUMAN SMCR7-like protein Smcr7l Smith Magenis syndrome chromosome region candidate gene 7 protein like Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like Smith-Magenis syndrome chromosome region, candidate 7-like |
Images
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Fig1:
Western blot analysis of SMCR7L / MID51 on different lysates with Rabbit anti-SMCR7L / MID51 antibody (HA724474) at 1/5,000 dilution. Lane 1: LNCaP (Human prostate cancer cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: 293T (Human embryonic kidney cell) cell lysate Lane 4: K-562 (Human chronic myelogenous leukemia ) cell lysate Lane 5: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724474, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 48-51 kDa |
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Fig2:
Western blot analysis of SMCR7L / MID51 on different lysates with Rabbit anti-SMCR7L / MID51 antibody (HA724474) at 1/5,000 dilution. Lane 1: Vero (African green monkey normal kidney cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lane 3: 4T1 (Mouse breast cancer cell) cell lysate Lane 4: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 6: C6 (Rat glioma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724474, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 48-51 kDa |
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Fig3:
Western blot analysis of SMCR7L / MID51 on different lysates with Rabbit anti-SMCR7L / MID51 antibody (HA724474) at 1/5,000 dilution.
Lane 1: Mouse testis tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat testis tissue lysate Lane 4: Pig brain tissue lysate Lane 5: Pig cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724474, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 51 kDa |
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Fig4:
Immunoprecipitation (IP)
SMCR7L / MID51 was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724474 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724474 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA724474 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA724474 in HeLa cell lysate Exposure time: 17 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724474, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 51 kDa Observed band size: 51 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.