CEMIP Recombinant Rabbit Monoclonal Antibody [PSH24-78]
cat.: HA724475
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 153 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CEMIP aa 1-450. |
| Positive control: | Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, Pig brain tissue lysate, Pig cerebellum tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Endoplasmic reticulum, Cell membrane, Membrane, clathrin-coated pit, Secreted. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q8WUJ3 Human | Q8BI06 Mouse Entrez Gene: 308797 Rat |
| Alternative names: | Cell migration-inducing and hyaluronan-binding protein EC:3.2.1.35 Hyaluronan binding protein involved in hyaluronan depolymerization CEMIP HYBID KIAA1199 |
Images
|
Fig1:
Western blot analysis of CEMIP on different lysates with Rabbit anti-CEMIP antibody (HA724475) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat skeletal muscle tissue lysate Lane 7: Pig brain tissue lysate Lane 8: Pig cerebellum tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Negative expression of CEMIP protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724475, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 153 kDa Observed band size: 150 kDa |
|
Fig2:
Immunoprecipitation (IP)
CEMIP was immunoprecipitated in 0.2 mg mouse brain tisue lysate with HA724475 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724475 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tisue lysate (input) Lane 2: HA724475 IP in Mouse brain tisue lysate Lane 3: Rabbit IgG instead of HA724475 in Mouse brain tisue lysate Exposure time: 2 minutes 6 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724475, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 153 kDa Observed band size: 150 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.