CXCL11 Recombinant Rabbit Monoclonal Antibody [PSH24-81]
cat.: HA724476
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CXCL11 aa 1-94. |
| Positive control: | THP-1 treated with 200ng/mL IFN-γ and 50ng/mlLLPS for 24 hours then add 300ng/mL BFA for last 20 hours cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IP |
1:5,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: O14625 Human |
| Alternative names: | C-X-C motif chemokine 11 Beta-R1 H174 Interferon gamma-inducible protein 9 (IP-9) Interferon-inducible T-cell alpha chemoattractant (I-TAC) Small-inducible cytokine B11 CXCL11 ITAC SCYB11 SCYB9B |
Images
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Fig1:
Western blot analysis of CXCL11 on different lysates with Rabbit anti-CXCL11 antibody (HA724476) at 1/5,000 dilution. Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 2: THP-1 treated with 200ng/mL IFN-γ and 50ng/mlLLPS for 24 hours then add 300ng/mL BFA for last 20 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724476, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 10 kDa Observed band size: 10 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: THP-1 (Human acute monoblastic leukemia cell) treated with 200ng/mL IFN-γ and 50ng/mlLLPS for 24 hours then add 300ng/mL BFA for last 20 hours Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724476, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
Immunoprecipitation (IP)
CXCL11 was immunoprecipitated in 0.2 mg THP-1 (Human acute monoblastic leukemia cell) treated with 200ng/mL IFN-γ and 50ng/mlLLPS for 24 hours then add 300ng/mL BFA for last 20 hours cell lysate with HA724476 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724476 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 treated with 200ng/mL IFN-γ and 50ng/mlLLPS for 24 hours then add 300ng/mL BFA for last 20 hours cell lysate (input) Lane 2: HA724476 IP in THP-1 treated with 200ng/mL IFN-γ and 50ng/mlLLPS for 24 hours then add 300ng/mL BFA for last 20 hours cell lysate Lane 3: Rabbit IgG instead of HA724476 in THP-1 treated with 200ng/mL IFN-γ and 50ng/mlLLPS for 24 hours then add 300ng/mL BFA for last 20 hours cell lysate Exposure time: 5 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724476, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 10 kDa Observed band size: 10 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.