Claudin 3 Recombinant Rabbit Monoclonal Antibody [PSH24-82]
cat.: HA724477
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-82 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Claudin 3 aa 181-220. |
| Positive control: | NCI-H358 (Human lung adenocarcinoma cells) cell lysate, HT-29 (Human colorectal adenocarcinoma cells) cell lysate, T-47D (Human breast ductal carcinoma cells) cell lysate, MCF7 cell lysate, Mouse colon tissue lysate, Rat colon tissue lysate. |
| Subcellular location: | Cell junction, tight junction, Cell membrane. |
| Recommended Dilutions:
WB IF-Tissue IP |
1:50,000 1:200-1:20,000 1-2μg/sample |
| Uniprot #: | SwissProt: O15551 Human | Q9Z0G9 Mouse | Q63400 Rat |
| Alternative names: | C7orf1 Claudin-3 Claudin3 CLD3_HUMAN CLDN 3 Cldn3 Clostridium perfringens enterotoxin receptor 2 CPE R2 CPE receptor 2 CPE-R 2 CPE-receptor 2 CPETR 2 CPETR2 HRVP 1 HRVP1 Rat ventral prostate 1 like protein Rat ventral prostate.1 protein homolog RVP1 Ventral prostate.1 like protein Ventral prostate.1 protein homolog |
Images
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Fig1:
Western blot analysis of Claudin 3 on different lysates with Rabbit anti-Claudin 3 antibody (HA724477) at 1/50,000 dilution.
Lane 1: NCI-H358 (Human lung adenocarcinoma cells) cell lysate Lane 2: Saos-2 (Human osteosarcoma cells) cell lysate Lane 3: HT-29 (Human colorectal adenocarcinoma cells) cell lysate Lane 4: T-47D (Human breast ductal carcinoma cells) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Low expression of Claudin 3 protein in Saos-2 cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724477, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 23 kDa Observed band size: 20 kDa |
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Fig2:
Western blot analysis of Claudin 3 on different lysates with Rabbit anti-Claudin 3 antibody (HA724477) at 1/50,000 dilution.
Lane 1: MCF7-si NT cell lysate Lane 2: MCF7-si Claudin 3 cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724477, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 23 kDa Observed band size: 20 kDa |
|
Fig3:
Western blot analysis of Claudin 3 on different lysates with Rabbit anti-Claudin 3 antibody (HA724477) at 1/50,000 dilution.
Lane 1: Mouse colon tissue lysate Lane 2: Mouse heart tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 seconds; ECL: K1801 Heart is a negative control (PMID: 9892664). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724477, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 23 kDa Observed band size: 20 kDa |
|
Fig4:
Western blot analysis of Claudin 3 on different lysates with Rabbit anti-Claudin 3 antibody (HA724477) at 1/50,000 dilution.
Lane 1: Rat colon tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Heart is a negative control (PMID: 9892664). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724477, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 23 kDa Observed band size: 20 kDa |
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Fig5:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724477, 1/20,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig6:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724477, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig7:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724477, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig8:
Immunoprecipitation (IP)
Claudin 3 was immunoprecipitated in 0.2 mg MCF7 (Human breast cancer cells) cell lysate with HA724477 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724477 at 1/20,000 dilution. Alpaca anti-Rabbit IgG Fc, Recombinant VHH monoclonal Antibody(HA1031) at 1/50,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: HA724477 IP in MCF7 cell lysate Lane 3: Rabbit IgG instead of HA724477 in MCF7 cell lysate Exposure time: 2 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724477, 1/20,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 23 kDa Observed band size: 20 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.