RYR1 Recombinant Rabbit Monoclonal Antibody [PSH24-83]
cat.: HA724478
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH24-83 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 565 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse RYR1 aa 1,301-1,450. |
| Positive control: | Mouse skeletal muscle tissue lysate, Mouse cerebellum tissue lysate, Rat skeletal muscle tissue lysate, Rat cerebellum tissue lysate, rat skeletal muscle tissue lysates. |
| Subcellular location: | Sarcoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:10,000 1:500 1:200-1:1,000 |
| Uniprot #: | SwissProt: E9PZQ0 Mouse | F1LMY4 Rat |
| Alternative names: | Arrhythmogenic right ventricular dysplasia 2 ARVC2 ARVD2 Brain ryanodine receptor calcium release channel Brain type ryanodine receptor Cardiac muscle ryanodine receptor calcium release channel Cardiac muscle type ryanodine receptor CCO Central core disease of muscle HBRR hRYR 2 hRYR2 MHS MHS1 Ryanodine receptor 1 (skeletal) Ryanodine receptor 1 Ryanodine receptor 2 (cardiac) Ryanodine receptor 2 Ryanodine receptor 3 Ryanodine receptor type1 RYDR RYR 1 RYR 2 RYR 3 RYR RYR-1 RyR1 RYR1_HUMAN RYR2 RYR3 Sarcoplasmic reticulum calcium release channel Skeletal muscle calcium release channel Skeletal muscle ryanodine receptor Skeletal muscle type ryanodine receptor Skeletal muscle-type ryanodine receptor SKRR Type 1 like ryanodine receptor Type 1 ryanodine receptor VTSIP |
Images
|
Fig1:
Western blot analysis of RYR1 on different lysates with Rabbit anti-RYR1 antibody (HA724478) at 1/10,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Rat skeletal muscle tissue lysate Lane 5: Rat liver tissue lysate Lane 6: Rat cerebellum tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Negative control: liver (PMID:7876312) . Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724478, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 565 kDa Observed band size: 500 kDa |
|
Fig2:
Western blot analysis of RYR1 on rat skeletal muscle tissue lysate with Rabbit anti-RYR1 antibody (HA724478) at 1/10,000 dilution.
Lysates/proteins at 30 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724478, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 565 kDa Observed band size: 500 kDa |
|
Fig3:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Skeletal muscle Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA724478, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunofluorescence (IHC-Fr)
Species: Rat Tissue: Skeletal muscle Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA724478, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724478, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724478, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724478, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724478, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative control: liver (PMID:7876312). |
|
Fig9:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724478, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig10:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724478, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig11:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724478, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative control: liver (PMID:7876312). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.