Cadherin 3 Recombinant Rabbit Monoclonal Antibody [PSH24-84]
cat.: HA724479
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH24-84 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Cadherin 3 aa 51-647. |
| Positive control: | A431 (Human epidermoid carcinoma skin squamous cells) cell lysate, BxPC-3 (Human primary pancreatic adenocarcinoma cells) cell lysate, Mouse placenta (E17) tissue lysate, Mouse embryo tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:25,000 1:200-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P22223 Human | P10287 Mouse | 116777 Rat |
| Alternative names: | Cadherin-3 Placental cadherin (P-cadherin) CDH3 CDHP |
Images
|
Fig1:
Western blot analysis of Cadherin 3 on different lysates with Rabbit anti-Cadherin 3 antibody (HA724479) at 1/25,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate (15 µg/Lane) Lane 2: HUVEC (Human umbilical vein endothelial cells) cell lysate (15 µg/Lane) Lane 3: BxPC-3 (Human primary pancreatic adenocarcinoma cells) cell lysate (15 µg/Lane) Lane 4: Mouse placenta (E17) tissue lysate (30 µg/Lane) Lane 5: Mouse embryo tissue lysate (30 µg/Lane) Exposure time: 4 seconds; ECL: K1801 Negative expression of Cadherin 3 protein in HUVEC cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724479, 1/25,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 91 kDa Observed band size: 120 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Placenta Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724479, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Breast Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724479, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724479, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Flow Cytometry
Species: Human Sample: A431 (Human epidermoid carcinoma skin squamous cells)/HUVEC (Human umbilical vein endothelial cells) Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724479 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative expression of Cadherin 3 protein in HUVEC cell is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.