Mouse/Rat RAGE Recombinant Rabbit Monoclonal Antibody [PSH24-86]
cat.: HA724481
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-86 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse RAGE aa 23-342 (HA211241). |
| Positive control: | Recombinant Mouse RAGE protein (HA211241). |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-85] to Mouse/Rat RAGE (Capture) (HA724480) and Recombinant Mouse RAGE protein (HA211241) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: Q62151 Mouse | Q63495 Rat |
| Alternative names: | Advanced glycosylation end product-specific receptor Ager MGC2235 Receptor for advanced glycosylation end products |
Images
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Fig1:
Sandwich ELISA analysis of Mouse RAGE matched pair antibodies Capture: HA724480, Mouse/Rat RAGE Rabbit mAb [PSH24-85] Detector: HA724481, Mouse/Rat RAGE Rabbit mAb [PSH24-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724480) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse RAGE protein (HA211241) starting from 2,000 pg/mL to 0 pg/mL and detect antibody (HA724481, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native RAGE in mouse serum and rat serum samples. Capture: HA724480, Mouse/Rat RAGE Rabbit mAb [PSH24-85] Detector: HA724481, Mouse/Rat RAGE Rabbit mAb [PSH24-86] The concentrations of RAGE were measured in duplicates, interpolated from the RAGE standard curve and corrected for sample dilution. Undiluted samples are mouse serum 100% and rat serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RAGE concentration was determined to be 1,853 pg/ml in mouse serum and 2,641 pg/ml in rat serum. |
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Fig3:
Interpolated concentrations of spiked RAGE in mouse cell culture media samples. Capture: HA724480, Mouse/Rat RAGE Rabbit mAb [PSH24-85] Detector: HA724481, Mouse/Rat RAGE Rabbit mAb [PSH24-86] The concentrations of RAGE were measured in duplicates, interpolated from the RAGE standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.