Biotin Conjugated Rat Prolactin Recombinant Rabbit Monoclonal Antibody [PSH24-88]
cat.: HA724483B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH24-88 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat Prolactin aa 30-266 (HA211351). |
| Positive control: | Recombinant Rat Prolactin protein (HA211351). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-87] to Rat Prolactin (Capture) (HA724482) and Recombinant Rat Prolactin protein (HA211351) as the standard. The reference range value is 7.8-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01237 Rat |
| Alternative names: | Decidual prolactin GHA1 Growth hormone A1 Lactogenic hormone Luteotropic hormone Mammotropin PRL Prolactin Prolactin precursor |
Images
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Fig1:
Sandwich ELISA analysis of Rat Prolactin matched pair antibodies Capture: HA724482, Rat Prolactin Rabbit mAb [PSH24-87] Detector: HA724483, Rat Prolactin Rabbit mAb [PSH24-88] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724482) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat Prolactin protein (HA211351) starting from 2,000 pg/mL to 0 pg/mL and detect antibody (HA724483, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Prolactin in rat serum samples. Capture: HA724482, Rat Prolactin Rabbit mAb [PSH24-87] Detector: HA724483, Rat Prolactin Rabbit mAb [PSH24-88] Interpolated concentration of native Prolactin was measured in duplicate at different sample concentrations and interpolated from the Prolactin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 12,020 pg/mL in rat serum. |
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Fig3:
Interpolated concentrations of native Prolactin in rat pituitary tissue extract samples based on a 1,000 µg/ml extract load. Capture: HA724482, Rat Prolactin Rabbit mAb [PSH24-87] Detector: HA724483, Rat Prolactin Rabbit mAb [PSH24-88] Interpolated concentration of native Prolactin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 127.4 μg/mL in rat pituitary tissue extract. |
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Fig4:
Interpolated concentrations of spiked Prolactin in cell culture media samples. Capture: HA724482, Rat Prolactin Rabbit mAb [PSH24-87] Detector: HA724483, Rat Prolactin Rabbit mAb [PSH24-88] The concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.