Biotin Conjugated Mouse/Rat IL-12 p40 Recombinant Rabbit Monoclonal Antibody [PSH24-92]
cat.: HA724487B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH24-92 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat IL-12 p40 aa 23-335 (HA211346). |
| Positive control: | Recombinant Rat IL-12 p40 protein (HA211346). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-94] to Mouse/Rat IL-12 p40 (Capture) (HA724489) and Recombinant Rat IL-12 p40 protein (HA211346) as the standard. The reference range value is 11.7-3,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P43432 Mouse | E9PU71 Rat |
| Alternative names: | Interleukin-12 subunit beta IL-12B Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40) IL-12 subunit p40 Il12b IL12B |
Images
|
Fig1:
Sandwich ELISA analysis of Rat IL-12 p40 matched pair antibodies Capture: HA724489, Mouse/Rat IL-12 p40 Rabbit mAb [PSH24-94] Detector: HA724487, Mouse/Rat IL-12 p40 Rabbit mAb [PSH24-92] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724489) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IL-12 p40 protein (HA211346) starting from 3,000 pg/mL to 0 pg/mL and detect antibody (HA724487, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IL-12 p40 in rat serum and mouse serum samples. Capture: HA724489, Mouse/Rat IL-12 p40 Rabbit mAb [PSH24-94] Detector: HA724487, Mouse/Rat IL-12 p40 Rabbit mAb [PSH24-92] Interpolated concentration of native IL-12 p40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-12 p40 concentration was determined to be 1,614 pg/mL in rat serum and 1,568 pg/mL in mouse serum. |
|
Fig3:
Interpolated concentrations of spiked IL-12 p40 in cell culture media samples. Capture: HA724489, Mouse/Rat IL-12 p40 Rabbit mAb [PSH24-94] Detector: HA724487, Mouse/Rat IL-12 p40 Rabbit mAb [PSH24-92] The concentrations of IL-12 p40 were measured in duplicates, interpolated from the IL-12 p40 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.