ALK Recombinant Rabbit Monoclonal Antibody [PSH24-98]
cat.: HA724493
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH24-98 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 176 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ALK aa 1,321-1,620. |
| Positive control: | SH-SY5Y (Human neuroblastoma cell) cell lysate, IMR-32 (Human neuroblastoma cell) cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:100-1:200 |
| Uniprot #: | SwissProt: Q9UM73 Human |
| Alternative names: | Alk ALK tyrosine kinase receptor ALK/EML4 fusion gene, included ALK/NPM1 fusion gene, included ALK_HUMAN anaplastic lymphoma kinase (Ki-1) Anaplastic lymphoma kinase Anaplastic lymphoma kinase Ki1 anaplastic lymphoma receptor tyrosine kinase CD 246 CD246 CD246 antigen EC 2.7.10.1 Ki 1 Ki1 mutant anaplastic lymphoma kinase NBLST 3 NBLST3 Tcrz TFG/ALK |
Images
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Fig1:
Western blot analysis of ALK on different lysates with Rabbit anti-ALK antibody (HA724493) at 1/5,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 3: Ramos (Human Burkitt's lymphoma cell) cell lysate Lane 4: IMR-32 (Human neuroblastoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Raji is a negative control (PMID: 30541790). Ramos is a negative control (PMID: 30541790). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724493, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 176 kDa Observed band size: 140-250 kDa |
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Fig2:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Anaplastic large cell lymphoma (ALCL) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724493, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Appendix (myenteric plexus) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724493, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Appendix Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724493, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lung adenocarcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724493, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of ALK protein in lung adenocarcinoma is consistent with the predicted expression pattern. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: SH-SY5Y (Human neuroblastoma cell) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724493, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Raji (Human Burkitt's lymphoma cell) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724493, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Raji is a negative control (PMID: 30541790). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.