Neurofibromin Recombinant Rabbit Monoclonal Antibody [PSH25-01]
cat.: HA724496
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH25-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 319 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Neurofibromin aa 231-580. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate. |
| Subcellular location: | Nucleus, nucleolus, Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P21359 Human | Q04690 Mouse | P97526 Rat |
| Alternative names: | DKFZp686J1293 FLJ21220 Neurofibromatosis Noonan syndrome Neurofibromatosis related protein NF 1 Neurofibromatosis related protein NF1 neurofibromatosis type I Neurofibromatosis-related protein NF-1 Neurofibromin 1 Neurofibromin truncated Neurofibromin1 NF 1 NF NF1 NF1_HUMAN NFNS Type 1 Neurofibromatosis von Recklinghausen disease neurofibromin von Recklinghausen disease related protein VRNF VRNF WATS Watson disease related protein WSS Watson syndrome WSS |
Images
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Fig1:
Western blot analysis of Neurofibromin on different lysates with Rabbit anti-Neurofibromin antibody (HA724496) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lane 3: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724496, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 319 kDa Observed band size: 300/250 kDa |
|
Fig2:
Western blot analysis of Neurofibromin on different lysates with Rabbit anti-Neurofibromin antibody (HA724496) at 1/5,000 dilution.
Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Neurofibromin cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724496, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 319 kDa Observed band size: 300/250 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.