CRTC1 Recombinant Rabbit Monoclonal Antibody [PSH25-02]
cat.: HA724497
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC(Intra), IP |
| Clonality: | Monoclonal |
| Clone number: | PSH25-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CRTC1 aa 251-500. |
| Positive control: | Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, HT22 (Mouse hippocampal neuron cell) cell lysate, C6 (Rat glioma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, SH-SY5Y (Human neuroblastoma cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, Mouse embryo tissue lysate, E14.0 mouse embryonic brain tissue lysate, Mouse brain (4weeks) tissue lysate, Mouse brain tissue lysate, Rat brain (4weeks) tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB FC(Intra) IP |
1:5,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q6UUV9 Human | Q68ED7 Mouse | Q157S1 Rat |
| Alternative names: | KIAA0616 CREB regulated transcription coactivator 1 CREB-regulated transcription coactivator 1 CRTC1 CRTC1_HUMAN FLJ14027 MECT 1 Mucoepidermoid carcinoma translocated 1 Mucoepidermoid carcinoma translocated protein 1 TORC-1 TORC1 Transducer of CREB protein 1 Transducer of regulated cAMP response element binding protein 1 Transducer of regulated cAMP response element-binding protein (CREB) 1 Transducer of regulated cAMP response element-binding protein 1 WAMTP1 |
Images
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Fig1:
Western blot analysis of CRTC1 on different lysates with Rabbit anti-CRTC1 antibody (HA724497) at 1/5,000 dilution.
Lane 1: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate Lane 2: HT22 (Mouse hippocampal neuron cell) cell lysate Lane 3: C6 (Rat glioma cell) cell lysate Lane 4: 293T (Human embryonic kidney cell) cell lysate Lane 5: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724497, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 67 kDa Observed band size: 67 kDa |
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Fig2:
Western blot analysis of CRTC1 on different lysates with Rabbit anti-CRTC1 antibody (HA724497) at 1/5,000 dilution.
Lane 1: Mouse embryo tissue lysate Lane 2: E14.0 mouse embryonic brain tissue lysate Lane 3: Mouse brain (4weeks) tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain (4weeks) tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 10 seconds; ECL: K1801 In total brain lysates, the expression oftorc1 mRNA increased after birth, peaked at P14, and decreased slightly in adulthood. (PMID: 19244510). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724497, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 67 kDa Observed band size: 67 kDa |
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Fig3:
Application: Flow Cytometry (Intra)
Species: Human Sample: 293T (Human embryonic kidney cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724497(1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig4:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: Neuro-2a (Mouse brain neuroblastoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724497(1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig5:
Immunoprecipitation (IP)
CRTC1 was immunoprecipitated in 0.2 mg Neuro-2a (Mouse brain neuroblastoma cell) cell lysate with HA724497 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724497 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Neuro-2A cell lysate (input) Lane 2: HA724497 IP in Neuro-2A cell lysate Lane 3: Rabbit IgG instead of HA724497 in Neuro-2A cell lysate Exposure time: 17 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724497, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 67 kDa Observed band size: 78 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.