Human/Monkey Pro-COL1A1 Recombinant Rabbit Monoclonal Antibody [PSH25-15]
cat.: HA724509
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rhesus monkey |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH25-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Pro-COL1A1 aa 23-161 (HA211375). |
| Positive control: | Recombinant Human Pro-COL1A1 protein (HA211375). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH25-16] to Human/Monkey Pro-COL1A1 (Detecor) (HA724510) and Recombinant Human Pro-COL1A1 protein (HA211375) as the standard. The reference range value is 19.5-2,500 pg/mL. |
| Uniprot #: | SwissProt: P02452 Human |
| Alternative names: | Alpha 1 type I collagen alpha1(I) procollagen CO1A1_HUMAN COL1A1 collagen alpha 1 chain type I Collagen alpha-1(I) chain collagen alpha-1(I) chain preproprotein Collagen I alpha 1 polypeptide collagen of skin, tendon and bone, alpha-1 chain Collagen type I alpha 1 EDSC OI1 OI2 OI3 OI4 pro-alpha-1 collagen type 1 type I proalpha 1 type I procollagen alpha 1 chain Type I procollagen |
Images
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Fig1:
Sandwich ELISA analysis of Human Pro-COL1A1 matched pair antibodies Capture: HA724509, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-15] Detector: HA724510, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-16] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724509) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Pro-COL1A1 protein (HA211375) starting from 2,500 pg/mL to 0 pg/mL and detect antibody (HA724510, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Pro-COL1A1 in human and monkey serum. Capture: HA724509, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-15] Detector: HA724510, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-16] Interpolated concentration of native Pro-COL1A1 was measured in duplicate at different sample concentrations and interpolated from the Pro-COL1A1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-COL1A1 concentration was determined to be 122,731 pg/mL in rhesus monkey serum and 25,357 pg/mL in human serum. |
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Fig3:
Interpolated concentrations of spiked Pro-COL1A1 in cell culture media samples. Capture: HA724509, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-15] Detector: HA724510, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-16] The concentrations of Pro-COL1A1 were measured in duplicates, interpolated from the Pro-COL1A1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.