Biotin Conjugated Human MUC6 Recombinant Rabbit Monoclonal Antibody [PSH25-19]
cat.: HA724513B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH25-19 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MUC6 aa 23-579 (HA211305). |
| Positive control: | Recombinant Human MUC6 protein (HA211305). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH25-17] to Human MUC6 (Capture) (HA724511) and Recombinant Human MUC6 protein (HA211305) as the standard. The reference range value is 78.1-20,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q6W4X9 Human |
| Alternative names: | Mucin-6 MUC-6 Gastric mucin-6 MUC6 |
Images
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Fig1:
Sandwich ELISA analysis of Human MUC6 matched pair antibodies Capture: HA724511, Human MUC6 Rabbit mAb [PSH25-17] Detector: HA724513, Human MUC6 Rabbit mAb [PSH25-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724511) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MUC6 protein (HA211305) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (HA724513, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of spiked MUC6 in human cell culture media samples. Capture: HA724511, Human MUC6 Rabbit mAb [PSH25-17] Detector: HA724513, Human MUC6 Rabbit mAb [PSH25-19] The concentrations of MUC6 were measured in duplicates, interpolated from the MUC6 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.