CD86 Recombinant Rabbit Monoclonal Antibody [PSH25-20]
cat.: HA724514
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH25-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CD86 aa 1-265. |
| Positive control: | Mouse spleen tissue lysate. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:5,000 1:200 1:200-1:1,000 |
| Uniprot #: | SwissProt: P42082 Mouse |
| Alternative names: | Activation B7-2 antigen 3 Activation B7-2 antigen B-lymphocyte activation antigen B7-2 2 B-lymphocyte activation antigen B7-2 B7 2 B7 B7-2 B7.2 B70 B72 B72 antigen BU63 CD28 antigen ligand 2 2 CD28 antigen ligand 2 Cd28l2 CD28LG2 CD86 CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) CD86 antigen CD86 molecule CD86_HUMAN CLS1 CTLA-4 counter-receptor B7.2 2 CTLA-4 counter-receptor B7.2 2, 3 CTLA-4 counter-receptor B7.2 Early T-cell costimulatory molecule 1 ETC-1 FUN 1 FUN-1 FUN1 LAB72 Ly-58 Ly58 MB7 MB7-2 Membrane glycoprotein MGC34413 T lymphocyte activation antigen CD86 precursor T-lymphocyte activation antigen CD86 TS/A-2 |
Images
|
Fig1:
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (HA724514) at 1/5,000 dilution.
Lane 1: Mouse spleen tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1802 Negative expression of CD86 protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724514, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 35 kDa Observed band size: 60-85 kDa |
|
Fig2:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Antibody A: 1/200 (CD86, HA724514, rabbit, Red), Antibody B: 1/200 (CD68, HA601596, rat, Green), overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 594, HA1122), Goat anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724514, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724514, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724514, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of CD86 protein in skeletal muscle is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.