GATA3 Recombinant Rabbit Monoclonal Antibody [PSH25-24]
cat.: HA724518
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH25-24 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa. |
| Isotype: | IgG |
| Positive control: | SH-SY5Y (Human neuroblastoma cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, MCF7 (Human breast cancer cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:100 1:200-1:500 |
| Uniprot #: | SwissProt: P23771 Human |
| Alternative names: | GATA 3 GATA binding factor 3 GATA binding protein 3 GATA-binding factor 3 Gata3 GATA3_HUMAN HDR HDRS MGC2346 MGC5199 MGC5445 Trans acting T cell specific transcription factor GATA 3 Trans-acting T-cell-specific transcription factor GATA-3 |
Images
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Fig1:
Western blot analysis of GATA3 on different lysates with Rabbit anti-GATA3 antibody (HA724518) at 1/5,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate Lane 4: MDA-MB-231 (Human breast cancer cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Low expression of GATA3 protein in MDA-MB-231 is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724518, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: SH-SY5Y (Human neuroblastoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724518, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Urothelial carcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724518, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724518, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.