GPI Recombinant Rabbit Monoclonal Antibody [PSH25-26]
cat.: HA724519
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH25-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, HCT 116 (Human colon cancer cell) cell lysate, PC-3 (Human prostate cancer cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse liver tissue lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:500-1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P06744 Human | P06745 Mouse | Q6P6V0 Rat |
| Alternative names: | AMF Aurocrine motility factor Autocrine motility factor DKFZp686C13233 EC 5.3.1.9 G6PI_HUMAN Glucose phosphate isomerase Glucose-6-phosphate isomerase GNPI GPI Gpi1 Hexose monophosphate isomerase Hexosephosphate isomerase Neuroleukin NLK Oxoisomerase PGI PHI Phosphoglucose isomerase Phosphohexomutase Phosphohexose isomerase Phosphosaccharomutase SA 36 SA-36 SA36 |
Images
|
Fig1:
Western blot analysis of GPI on different lysates with Rabbit anti-GPI antibody (HA724519) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: HCT 116 (Human colon cancer cell) cell lysate Lane 3: PC-3 (Human prostate cancer cell) cell lysate Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate Lane 5: A549 (Human lung adenocarcinoma cell) cell lysate Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724519, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 63 kDa Observed band size: 55 kDa |
|
Fig2:
Western blot analysis of GPI on different lysates with Rabbit anti-GPI antibody (HA724519) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat liver tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724519, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 63 kDa Observed band size: 55 kDa |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724519, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Kindey Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724519, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724519, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kindey Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724519, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724519, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig8:
Immunoprecipitation (IP)
GPI was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724519 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724519 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA724519 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA724519 in HeLa cell lysate Exposure time: 3 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724519, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 63 kDa Observed band size: 55 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.