HRP Conjugated Human/Monkey IGFBP2 Recombinant Rabbit Monoclonal Antibody [PSH25-34]
cat.: HA724525H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH25-34 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IGFBP2 aa 36-325 (HA211103). |
| Positive control: | Recombinant Human IGFBP2 protein (HA211103). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH25-33] to Human/Monkey IGFBP2 (Capture) (HA724524) and Recombinant Human IGFBP2 protein (HA211103) as the standard. The reference range value is 312.5-40,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P18065 Human |
| Alternative names: | BP 2 BP2 IBP 2 IBP-2 IBP2 IBP2_HUMAN IGF binding protein 2 IGF BP53 IGF-binding protein 2 IGFBP 2 IGFBP-2 IGFBP2 IGFBP53 Insulin like growth factor binding protein 2 36kDa Insulin like growth factor binding protein 2 Insulin like growth factor-binding protein 2 precursor Insulin-like growth factor-binding protein 2 |
Images
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Fig1:
Sandwich ELISA analysis of Human IGFBP2 matched pair antibodies Capture: HA724524, Human/Monkey IGFBP2 Rabbit mAb [PSH25-33] Detector: HA724525, Human/Monkey IGFBP2 Rabbit mAb [PSH25-34] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724524) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IGFBP2 protein (HA211103) starting from 40,000 pg/mL to 0 pg/mL and detect antibody (HA724525, HRP, 0.05 μg/mL) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.