Phospho-Beta Catenin (S675) Recombinant Rabbit Monoclonal Antibody [PSH25-60]
cat.: HA724534
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH25-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser675 of Human Beta catenin. |
| Positive control: | Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IP |
1:5,000 1:1,000 1:4,000 1-2μg/sample |
| Uniprot #: | SwissProt: P35222 Human | Q02248 Mouse | Q9WU82 Rat |
| Alternative names: | b-catenin Beta catenin Beta-catenin Cadherin associated protein Catenin (cadherin associated protein), beta 1, 88kDa Catenin beta 1 Catenin beta-1 CATNB CHBCAT CTNB1_HUMAN CTNNB CTNNB1 DKFZp686D02253 FLJ25606 FLJ37923 OTTHUMP00000162082 OTTHUMP00000165222 OTTHUMP00000165223 OTTHUMP00000209288 OTTHUMP00000209289 |
Images
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Fig1:
Western blot analysis of Phospho-Beta Catenin (S675) on different lysates with Rabbit anti-Phospho-Beta Catenin (S675) antibody (HA724534) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lane 4: Rat brain tissue lysate Lane 5: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-3: 1 minute; Lane 4-5: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724534, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 85 kDa Observed band size: 85 kDa |
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Fig2:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Colon Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724534, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724534, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724534, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724534, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Immunoprecipitation (IP)
Phospho-Beta Catenin (S675) was immunoprecipitated in 0.2 mg mouse brain tissue lysate with HA724534 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724534 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA724534 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA724534 in mouse brain tissue lysate Exposure time: 25 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724534, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 85 kDa Observed band size: 85 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.