CerS2 Recombinant Rabbit Monoclonal Antibody [PSH25-62]
cat.: HA724549
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-Fr, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH25-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Positive control: | HepG2 (Human liver cancer cell) cell lysate, Huh7 (Human liver cancer cell) cell lysate, SH-SY5Y(Human neuroblastoma cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-Fr IHC-P IP |
1:2,000 1:500 1:200 1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q96G23 Human | Q924Z4 Mouse Entrez Gene: 310667 Rat |
| Alternative names: | Ceramide synthase 2 CerS 2 CerS2 CERS2_HUMAN FLJ10243 L3 LAG1 homolog ceramide synthase 2 LAG1 longevity assurance 2 LAG1 longevity assurance homolog 2 LASS 2 Longevity assurance (LAG1 S. cerevisiae) homolog 2 MGC987 SP 260 SP260 TMSG 1 TMSG1 Tumor metastasis suppressor Tumor metastasis suppressor gene 1 protein Tumor metastasis-suppressor gene 1 protein |
Images
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Fig1:
Western blot analysis of CerS2 on different lysates with Rabbit anti-CerS2 antibody (HA724549) at 1/2,000 dilution.
Lane 1: HepG2 (Human liver cancer cell) cell lysate Lane 2: Huh7 (Human liver cancer cell) cell lysate Lane 3: SH-SY5Y(Human neuroblastoma cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724549, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 40 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: Hep G2 (Human hepatocellar carcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724549, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Liver Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724549, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724549, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724549, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724549, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724549, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724549, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Skeletal muscle exhibits low expression levels of CerS2 (PMID: 18165233). |
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Fig9:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724549, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig10:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724549, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig11:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724549, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Skeletal muscle exhibits low expression levels of CerS2 (PMID: 18165233). |
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Fig12:
Immunoprecipitation (IP)
CerS2 was immunoprecipitated in 0.2 mg Hep G2 (Human hepatocellar carcinoma cell) cell lysate with HA724549 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724549 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Hep G2 cell lysate (input) Lane 2: HA724549 IP in Hep G2 cell lysate Lane 3: Rabbit IgG instead of HA724549 in Hep G2 cell lysate Exposure time: 20 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724549, 1/2,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 45 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.