Erk1 (pT202+pY204) + Erk2 (pT185+pY187) Recombinant Rabbit Monoclonal Antibody [PD00-38]
cat.: HA724560
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PD00-38 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41/43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human aa 166-215 / 379. |
| Positive control: | A431 cell lysate, SK-Br-3 cell lysate, human human thyroid carcinoma tissue, MCF-7. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:5,000 1:50 |
| Uniprot #: | SwissProt: P27361 Human | P28482 Human |
| Alternative names: | ERK 1 ERK 2 ERK-1 ERK-2 ERK1 erk1/2 ERK2 ERT1 ERT2 Extracellular signal regulated kinase 1 Extracellular signal-regulated kinase 1 Extracellular signal-regulated kinase 2 HS44KDAP HUMKER1A Insulin-stimulated MAP2 kinase MAP kinase 1 MAP kinase 2 MAP kinase 3 MAP kinase isoform p42 MAP kinase isoform p44 MAPK 1 MAPK 2 MAPK 3 Mapk1 MAPK2 MAPK3 Microtubule-associated protein 2 kinase Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 2 Mitogen-activated protein kinase 3 MK01_HUMAN p38 p40 p41 p41mapk p42-MAPK P42MAPK p44-ERK1 p44-MAPK p44ERK1 p44MAPK PRKM 2 PRKM1 PRKM2 PRKM3 protein tyrosine kinase ERK2 |
Images
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Fig1:
Western blot analysis of Erk1 (pT202+pY204) + Erk2 (pT185+pY187) on different lysates with Rabbit anti-Erk1 (pT202+pY204) + Erk2 (pT185+pY187) antibody (HA724560) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SK-Br-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41/43 kDa Observed band size: 41/43 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724560) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202+pY204) + Erk2 (pT185+pY187) antibody (HA724560) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724560) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of MCF-7 cells labeling Erk1 (pT202+pY204) + Erk2 (pT185+pY187) with Rabbit anti-Erk1 (pT202+pY204) + Erk2 (pT185+pY187) antibody (HA724560) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Erk1 (pT202+pY204) + Erk2 (pT185+pY187) antibody (HA724560) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.