iFluor™ 647 Conjugated CD63 Recombinant Rabbit Monoclonal Antibody [SY21-02]
cat.: HA724566F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IF-Tissue
Clonality: Monoclonal
Clone number: SY21-02
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human CD63 aa 88-216 / 238.
Positive control: Human spleen tissue.
Subcellular location: Lysosome membrane, Cell membrane, extracellular exosome, Late endosome membrane, multivesicular body, Melanosome, Cell surface.
Recommended Dilutions:
  IF-Tissue

1:50
Uniprot #: SwissProt: P08962 Human
Alternative names: Lysosomal associated membrane protein 3 CD 63 CD63 CD63 antigen (melanoma 1 antigen) CD63 antigen CD63 antigen melanoma 1 antigen CD63 molecule CD63_HUMAN gp55 Granulophysin LAMP 3 LAMP-3 LAMP3 LIMP Lysosomal-associated membrane protein 3 Lysosome associated membrane glycoprotein 3 Mast cell antigen AD1 ME491 Melanoma 1 antigen Melanoma associated antigen ME491 Melanoma associated antigen MLA1 Melanoma-associated antigen ME491 MGC72893 MLA 1 MLA1 NGA Ocular melanoma associated antigen Ocular melanoma-associated antigen OMA81H PTLGP40 Tetraspanin 30 Tetraspanin-30 Tspan 30 Tspan-30 TSPAN30
Images
HA724566F_1.jpg Fig1: Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling CD63 (HA724566F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody CD63 (HA724566F, iFluor™ 647) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.