| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SY21-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD63 aa 88-216 / 238. |
| Positive control: | Human spleen tissue. |
| Subcellular location: | Lysosome membrane, Cell membrane, extracellular exosome, Late endosome membrane, multivesicular body, Melanosome, Cell surface. |
| Recommended Dilutions:
IF-Tissue |
1:50 |
| Uniprot #: | SwissProt: P08962 Human |
| Alternative names: | Lysosomal associated membrane protein 3 CD 63 CD63 CD63 antigen (melanoma 1 antigen) CD63 antigen CD63 antigen melanoma 1 antigen CD63 molecule CD63_HUMAN gp55 Granulophysin LAMP 3 LAMP-3 LAMP3 LIMP Lysosomal-associated membrane protein 3 Lysosome associated membrane glycoprotein 3 Mast cell antigen AD1 ME491 Melanoma 1 antigen Melanoma associated antigen ME491 Melanoma associated antigen MLA1 Melanoma-associated antigen ME491 MGC72893 MLA 1 MLA1 NGA Ocular melanoma associated antigen Ocular melanoma-associated antigen OMA81H PTLGP40 Tetraspanin 30 Tetraspanin-30 Tspan 30 Tspan-30 TSPAN30 |
|
Fig1:
Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling CD63 (HA724566F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody CD63 (HA724566F, iFluor™ 647) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |