NUMB Recombinant Rabbit Monoclonal Antibody [PSH25-86]
cat.: HA724578
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Green monkey |
| Applications: | WB, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH25-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NUMB aa 301-650. |
| Positive control: | U-87 MG (Human glioblastoma cell) cell lysates, SK-OV-3 (Human ovarian cancer cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate. |
| Subcellular location: | Cell membrane, Endosome membrane. |
| Recommended Dilutions:
WB FC(Intra) |
1:10,000 1:1,000 |
| Uniprot #: | SwissProt: P49757 Human | Q2LC84 Rat |
| Alternative names: | c14_5527 C14orf41 FLJ31314 h Numb h-Numb Numb Numb homolog (Drosophila) Numb homolog Numb protein homolog NUMB_HUMAN Protein numb homolog Protein S171 S171 |
Images
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Fig1:
Western blot analysis of NUMB on U-87 MG (Human glioblastoma cell) cell lysate with Rabbit anti-NUMB antibody (HA724578) at 1/10,000 dilution.
Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724578, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 71 kDa Observed band size: 72/71/66/65 kDa |
|
Fig2:
Western blot analysis of NUMB on different lysates with Rabbit anti-NUMB antibody (HA724578) at 1/10,000 dilution.
Lane 1: SK-OV-3 (Human ovarian cancer cell) cell lysate Lane 2: SK-BR-3 (Human breast cancer cell) cell lysate Lane 3: COS-1 (African green monkey kidney fibroblast) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Negative control: SK-BR-3 (PMID: 20443831). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724578, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 71 kDa Observed band size: 72/71/66/65 kDa |
|
Fig3:
Application: Flow Cytometry (Intra) Species: Rat Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724578 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.