NDRG2 Recombinant Rabbit Monoclonal Antibody [PSH25-90]
cat.: HA724582
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH25-90 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NDRG2 aa 1-371. |
| Positive control: | HCT 116 (Human colon cancer cell) cell lysate, COLO205 (Human colon adenocarcinoma cell) cell lysate, LoVo (Human colon cancer cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, T-47D (Human breast ductal carcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, CTLL-2 (Mouse T lymphocyte) cell lysate. |
| Subcellular location: | Cytoplasm, perinuclear region, Cell projection, growth cone. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: Q9UN36 Human | Q9QYG0 Mouse | Q8VBU2 Rat |
| Alternative names: | Protein NDRG2 N-myc downstream-regulated gene 2 protein Protein Syld709613 NDRG2 KIAA1248 SYLD |
Images
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Fig1:
Western blot analysis of NDRG2 on different lysates with Rabbit anti-NDRG2 antibody (HA724582) at 1/5,000 dilution.
Lane 1: HCT 116 (Human colon cancer cell) cell lysate Lane 2: COLO205 (Human colon adenocarcinoma cell) cell lysate Lane 3: LoVo (Human colon cancer cell) cell lysate Lane 4: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lane 5: T-47D (Human breast ductal carcinoma cell) cell lysate Lane 6: MCF7 (Human breast cancer cell) cell lysate Lane 7: MDA-MB-231 (Human breast cancer cell) cell lysate Lane 8: CTLL-2 (Mouse T lymphocyte) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Low expression: MDA-MB-231 (PMID: 24636131) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724582, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 41 kDa Observed band size: 41 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: CTLL-2 (Mouse T lymphocyte) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724582, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724582, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724582, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.