GLA Recombinant Rabbit Monoclonal Antibody [PSH25-93]
cat.: HA724585
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH25-93 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GLA aa 1-429. |
| Positive control: | MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:100 1:200 1:1,000 |
| Uniprot #: | SwissProt: P06280 Human |
| Alternative names: | AGAL_HUMAN Agalsidase alfa Alpha D galactosidase A Alpha D galactoside galactohydrolase 1 Alpha D galactoside galactohydrolase Alpha gal A Alpha galactosidase A Alpha-D-galactosidase A Alpha-D-galactoside galactohydrolase Alpha-galactosidase A GALA Galactosidase, alpha GLA GLA protein Melibiase |
Images
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Fig1:
Western blot analysis of GLA on different lysates with Rabbit anti-GLA antibody (HA724585) at 1/5,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate Lane 5: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 6: LNCaP (Human prostate cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724585, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 49 kDa Observed band size: 49 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: MCF7 (Human breast cancer cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724585, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig3:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724585, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724585, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Flow Cytometry (Intra)
Species: Human Sample: MCF7 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724585 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.