Biotin Conjugated CREB1 Recombinant Rabbit Monoclonal Antibody [PSH26-25]
cat.: HA724611B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH26-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CREB1 aa 1-259 (HA211601). |
| Positive control: | Recombinant Mouse CREB1 protein (HA211601). |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [SA04-04] to CREB1 (Capture) (HA724610) and Recombinant CREB1 protein (HA211601) as the standard. The reference range value is 78.1-20,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P16220 Human | Q01147 Mouse | P15337 Rat |
| Alternative names: | Active transcription factor CREB cAMP response element binding protein 1 cAMP response element binding protein cAMP responsive element binding protein 1 cAMP-responsive element-binding protein 1 CREB CREB-1 CREB1 CREB1_HUMAN Cyclic AMP-responsive element-binding protein 1 MGC9284 OTTHUMP00000163864 OTTHUMP00000163865 OTTHUMP00000206660 OTTHUMP00000206662 OTTHUMP00000206667 Transactivator protein |
Images
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Fig1:
Sandwich ELISA analysis of CREB1 matched pair antibodies Capture: HA724610, CREB1 Rabbit mAb [SA04-04] Detector: HA724611, CREB1 Rabbit mAb [PSH26-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724610) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CREB1 protein (HA211601) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (HA724611, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CREB1 in HepG2, NIH/3T3 and C6 Cell extract samples based on a 1,000 µg/mL extract load. Capture: HA724610, CREB1 Rabbit mAb [SA04-04] Detector: HA724611, CREB1 Rabbit mAb [PSH26-25] Interpolated concentration of native CREB1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CREB1 concentration was determined to be 29,085 pg/mL in HepG2 Cell extract,23,515 pg/mL in NIH/3T3 Cell extract and 24,845 pg/mL in C6 Cell extract. |
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Fig3:
Interpolated concentrations of spiked CREB1 in cell culture media samples. Capture: HA724610, CREB1 Rabbit mAb [SA04-04] Detector: HA724611, CREB1 Rabbit mAb [PSH26-25] The concentrations of CREB1 were measured in duplicates, interpolated from the CREB1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.