SOD1 Recombinant Rabbit Monoclonal Antibody [PSH26-10]
cat.: HA724615
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Pig |
| Applications: | WB, IF-Cell, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH26-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within rat SOD1 aa 1-154. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, Hep G2 (Human liver cancer cell) cell lysate, HUVEC (Human umbilical vein endothelial cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C2C12 (Mouse myoblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Rat stomach tissue lysate, Pig brain tissue lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, RAW264.7 treated with 100ng/mL LPS for 24 hours cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC(Intra) |
1:5,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: P00441 Human | P08228 Mouse | P07632 Rat |
| Alternative names: | Cu/Zn SOD Cu/Zn superoxide dismutase SOD1 Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 Superoxide dismutase 1 soluble Superoxide dismutase Cu Zn |
Images
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Fig1:
Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA724615) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Hep G2 (Human liver cancer cell) cell lysate Lane 3: HUVEC (Human umbilical vein endothelial cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724615, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 16 kDa Observed band size: 16 kDa |
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Fig2:
Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA724615) at 1/5,000 dilution.
Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate Lane 3: C2C12 (Mouse myoblast) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724615, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 16 kDa Observed band size: 16 kDa |
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Fig3:
Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA724615) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat stomach tissue lysate Lane 6: Pig brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724615, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 16 kDa Observed band size: 16 kDa |
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Fig4:
Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA724615) at 1/5,000 dilution.
Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 24 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724615, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 16 kDa Observed band size: 16 kDa |
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Fig5:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: Neuro-2a (Mouse brain neuroblastoma cell) % Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724615, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig6:
Application: Flow Cytometry (Intra) Species: Mouse Sample: Neuro-2a (Mouse brain neuroblastoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724615 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.