HRP Conjugated Human c-Kit Recombinant Rabbit Monoclonal Antibody [PSH26-41]
cat.: HA724620H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH26-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human c-Kit aa 26-516 (HA211233). |
| Positive control: | Recombinant Human c-Kit protein (HA211233). |
| Subcellular location: | Cell membrane; Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-40] to Human c-Kit (Capture) (HA724619) and Recombinant Human c-Kit protein (HA211233) as the standard. The reference range value is 23.44-3,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10721 Human |
| Alternative names: | C Kit c-Kit c-Kit Ligand CD117 Kit Kit Ligand KIT oncogene KIT proto oncogene receptor tyrosine kinase KIT_HUMAN Mast cell growth factor receptor Mast/stem cell growth factor receptor Kit MGF p145 c-kit PBT Piebald trait protein Proto oncogene c Kit Proto oncogene tyrosine protein kinase Kit Proto-oncogene c-Kit SCF Receptor SCFR soluble KIT variant 1 Steel Factor Receptor Stem cell factor receptor tyrosine protein kinase Kit Tyrosine-protein kinase Kit v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
Images
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Fig1:
Sandwich ELISA analysis of Human c-Kit matched pair antibodies Capture: HA724619, Human c-Kit Rabbit mAb [PSH26-40] Detector: HA724620, Human c-Kit Rabbit mAb [PSH26-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724619) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human c-Kit protein (HA211233) starting from 3,000 pg/mL to 0 pg/mL and detect antibody (HA724620, HRP, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.