HLA-DR Recombinant Rabbit Monoclonal Antibody [PSH26-51]
cat.: HA724628
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH26-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HLA-DR aa 51-216. |
| Positive control: | Raji (Human Burkitt's lymphoma cell) cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus, Endosome membrane, Lysosome membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:500 1:9,000 |
| Uniprot #: | SwissProt: P01903 Human |
| Alternative names: | DR alpha chain DR alpha chain precursor DRA_HUMAN DRB1 DRB4 Histocompatibility antigen HLA DR alpha HLA class II histocompatibility antigen HLA class II histocompatibility antigen DR alpha chain HLA DR1B HLA DR3B HLA DRA HLA DRA1 HLA DRB1 HLA DRB3 HLA DRB4 HLA DRB5 HLA-DRA HLADR4B HLADRA1 HLADRB Major histocompatibility complex class II DR alpha Major histocompatibility complex class II DR beta 1 Major histocompatibility complex class II DR beta 3 Major histocompatibility complex class II DR beta 4 Major histocompatibility complex class II DR beta 5 MGC117330 MHC cell surface glycoprotein MHC class II antigen DRA MHC II MLRW |
Images
|
Fig1:
Western blot analysis of HLA-DR on different lysates with Rabbit anti-HLA-DR antibody (HA724628) at 1/5,000 dilution.
Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 3: Ramos (Human Burkitt's lymphoma cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Negative expression of HLA-DR protein in Jurkat cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724628, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 30-40 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: Raji (Human Burkitt's lymphoma cell)/Jurkat (Human T-lymphoblastic cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Negative expression of HLA-DR protein in Jurkat cell is consistent with the predicted expression pattern Primary antibody: HA724628, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Bladder adenocarcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724628, 1/9,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724628, 1/9,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724628, 1/9,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.