CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71]
cat.: HA724636
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH26-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Positive control: | Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate. |
| Subcellular location: | Endoplasmic reticulum. Plasma membrane. Cell projection. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:500-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: O43490 Human |
| Alternative names: | AC133 Antigen AC133 CD133 CORD12 Hematopoietic stem cell antigen hProminin MCDR2 MSTP061 OTTHUMP00000217744 OTTHUMP00000217745 OTTHUMP00000217746 PROM1 PROM1_HUMAN Prominin I Prominin like 1 Prominin like protein 1 precursor Prominin mouse like 1 Prominin-1 Prominin-like protein 1 Prominin1 PROML1 RP41 STGD4 |
Images
|
Fig1:
Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (HA724636) at 1/1,000 dilution.
Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lane 3: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 34 seconds; ECL: K1802 293T is a negative control (PMID: 30783186). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724636, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 97 kDa Observed band size: 120 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724636, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724636, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Flow Cytometry
Species: Human Sample: 293T (Human embryonic kidney cells) (Left, Negative control) / Caco-2 (Human colorectal adenocarcinoma cell) (Right) Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724636 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: 293T (PMID: 30783186). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.