CDCP1 Recombinant Rabbit Monoclonal Antibody [PSH26-74]
cat.: HA724639
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH26-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CDCP1 aa 1-667. |
| Positive control: | HCT 116 (Human colon cancer cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, COLO205 (Human colon adenocarcinoma cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, Mouse colon tissue lysate, Mouse small intestine tissue lysate, Rat colon tissue lysate, Rat small intestine tissue lysate. |
| Subcellular location: | Cell membrane. Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:5,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q9H5V8 Human | Q5U462 Mouse Entrez Gene: 301082 Rat |
| Alternative names: | 9030022E12Rik AA409659 CD 318 CD318 CD318 antigen CDCP 1 Cdcp1 CDCP1_HUMAN CUB domain containing protein 1 CUB domain-containing protein 1 E030027H19Rik FLJ13772 FLJ22375 FLJ22969 Membrane glycoprotein gp140 MGC31813 OTTHUMP00000164120 OTTHUMP00000209568 RGD1305578 SIMA 135 SIMA135 Subtractive immunization M plus HEp3 associated 135 kDa protein Subtractive immunization M plus HEp3-associated 135 kDa protein Transmembrane and associated with src kinases TRASK |
Images
|
Fig1:
Western blot analysis of CDCP1 on different lysates with Rabbit anti-CDCP1 antibody (HA724639) at 1/5,000 dilution.
Lane 1: HCT 116 (Human colon cancer cell) cell lysate (15 µg/Lane) Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate (15 µg/Lane) Lane 3: COLO205 (Human colon adenocarcinoma cell) cell lysate (15 µg/Lane) Lane 4: MDA-MB-231 (Human breast cancer cell) cell lysate (15 µg/Lane) Lane 5: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane) Lane 6: Mouse colon tissue lysate (20 µg/Lane) Lane 7: Mouse small intestine tissue lysate (20 µg/Lane) Lane 8: Rat colon tissue lysate (20 µg/Lane) Lane 9: Rat small intestine tissue lysate (20 µg/Lane) Exposure time: 25 seconds; ECL: K1801 Negative control: MCF7 (PMID: 20551327, 24055141). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724639, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 92.9 kDa Observed band size: 140, 70 kDa The molecular weight is consistent with the reference (PMID: 20551327). |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724639, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724639, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724639, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Flow Cytometry
Species: Human Sample: MCF7 (Human breast cancer cells) (Left, Negative control)/HCT 116 (Human colon cancer cells) (Right, Positive control) Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724639 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: MCF7 ((PMID: 20551327, 24055141). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.