IGF1R Recombinant Rabbit Monoclonal Antibody [PSH26-79]
cat.: HA724644
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH26-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 155 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IGF1R aa 550-935. |
| Positive control: | MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, HepG2 (Human hepatocellular carcinoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, C2C12 (Mouse myoblast) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000-1:20,000 1:200 1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P08069 Human | Q60751 Mouse | P24062 Rat |
| Alternative names: | Insulin-like growth factor 1 receptor beta chain CD221 CD221 antigen IGF 1 receptor IGF 1R IGF I receptor IGF-I receptor Igf1r IGF1R_HUMAN IGFIR IGFIRC IGFR Insulin like growth factor 1 receptor Insulin like growth factor 1 receptor precursor Insulin-like growth factor 1 receptor beta chain Insulin-like growth factor I receptor JTK13 MGC142170 MGC142172 MGC18216 Soluble IGF1R variant 1 Soluble IGF1R variant 2 |
Images
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Fig1:
Western blot analysis of IGF1R on different lysates with Rabbit anti-IGF1R antibody (HA724644) at 1/20,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: HepG2 (Human hepatocellular carcinoma cell) cell lysate Lane 4: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 5: C2C12 (Mouse myoblast) cell lysate Lane 6: NIH/3T3 (Mouse fibroblast) cell lysate Lane 7: C6 (Rat glioma cell) cell lysate The expression profile observed is consistent with what has been described in the literature (PMID: 11402025). Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724644, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 155 kDa Observed band size: 100/200 kDa |
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Fig2:
Western blot analysis of IGF1R on different lysates with Rabbit anti-IGF1R antibody (HA724644) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat brain tissue lysate The expression profile observed is consistent with what has been described in the literature (PMID: 11402025). Lysates/proteins at 30 µg/Lane. Exposure time: 1 minute; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724644, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 155 kDa Observed band size: 100/200 kDa |
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Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: MCF7 (Human breast cancer cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724644, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Bronchus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Breast Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of IGF1R protein in skeletal muscle is consistent with the predicted expression pattern. |
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Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig9:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Breast Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig10:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Stomach Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig11:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of IGF1R protein in skeletal muscle is consistent with the predicted expression pattern. |
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Fig12:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig13:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Breast Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig14:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Stomach Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig15:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724644, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of IGF1R protein in skeletal muscle is consistent with the predicted expression pattern. |
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Fig16:
Application: Flow Cytometry
Species: Human Sample: MCF7 (Human breast cancer cells) Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724644 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.