PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH27-00]
cat.: HA724661
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH27-00 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 123 kDa |
| Isotype: | IgG |
| Positive control: | A-204 (Human rhabdomyosarcoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate. |
| Subcellular location: | Cell membrane, Cell projection, cilium, Golgi apparatus. |
| Recommended Dilutions:
WB IF-Cell FC(Intra) |
1:5,000 1:50-1:100 1:1,000 |
| Uniprot #: | SwissProt: P16234 Human | P26618 Mouse | P20786 Rat |
| Alternative names: | Alpha-type platelet-derived growth factor receptor CD140 antigen-like family member A CD140a CD140a antigen MGC74795 PDGF alpha chain PDGF-R-alpha PDGFR 2 PDGFR alpha PDGFR2 PDGFRA PDGFRA/BCR fusion PGFRA_HUMAN Platelet derived growth factor receptor 2 Platelet derived growth factor receptor alpha Platelet derived growth factor receptor alpha polypeptide Platelet derived growth factor receptor Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein RHEPDGFRA |
Images
|
Fig1:
Western blot analysis of PDGFR alpha on different lysates with Rabbit anti-PDGFR alpha antibody (HA724661) at 1/5,000 dilution.
Lane 1: A-204 (Human rhabdomyosarcoma cell) cell lysate Lane 2: A-172 (Human brain glioblastoma cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds ; ECL: K1801 A172 is a negative control (PMID: 8425771). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724661, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 123 kDa Observed band size: 150-200 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: A-204 (Human rhabdomyosarcoma cell),/,A-172 (Human brain glioblastoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724661, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Negative control: A-172 (PMID: 8425771). |
|
Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: NIH/3T3 (Mouse fibroblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724661, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig4:
Application: Immunocytochemistry (IF-cell)
Species: Rat Sample: C6 (Rat glioma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724661, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig5:
Application: Flow Cytometry (Intra)
Species: Human Sample: A-172 (Human brain glioblastoma cell) (Left, Negative) / A-204 (Human rhabdomyosarcoma cell) (Right) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724661 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: A-172 (PMID: 8425771). |
|
Fig6:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724661 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
|
Fig7:
Application: Flow Cytometry (Intra)
Species: Rat Sample: C6 (Rat glioma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724661 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.