CD34 Recombinant Rabbit Monoclonal Antibody [PSH27-02]
cat.: HA724663
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-Fr, IHC-P
Clonality: Monoclonal
Clone number: PSH27-02
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 0.5ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 41 kDa
Isotype: IgG
Positive control: TF-1 (Human erythroleukemia cell) cell lysate.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-Fr
  IHC-P
1:1,000
1:100
1:250
1:2,500
Uniprot #: SwissProt: P28906 Human | Q64314 Mouse
Entrez Gene: 305081 Rat
Alternative names: CD34 CD34 antigen CD34 molecule CD34_HUMAN Cluster designation 34 cluster of differentiation 34 Hematopoietic progenitor cell antigen CD34 HPCA1 Mucosialin OTTHUMP00000034733 OTTHUMP00000034734
Images
HA724663_1.jpg Fig1: Western blot analysis of CD34 on different lysates with Rabbit anti-CD34 antibody (HA724663) at 1/1,000 dilution.

Lane 1: TF-1 (Human erythroleukemia cell) cell lysate
Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801

Negative control: Daudi (PMID: 11606408).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724663, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 41 kDa
Observed band size: 80-120 kDa
HA724663_2.jpg Fig2: Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: TF-1 (Human erythroleukemia cell) / Daudi (Human Burkitt's lymphoma cell) (negative)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA724663, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

Negative control: Daudi (PMID: 11606408).
HA724663_3.jpg Fig3: Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Kidney
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

Primary antibody: HA724663, 1/250, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
HA724663_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Cerebral cortex
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724663_5.jpg Fig5: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724663_6.jpg Fig6: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Cerebral cortex
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724663_7.jpg Fig7: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724663_8.jpg Fig8: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Cerebral cortex
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724663_9.jpg Fig9: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.