Phospho-IRF3 (S396) Recombinant Rabbit Monoclonal Antibody [PSH27-05]
cat.: HA724666
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH27-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser388 of moues IRF3. |
| Positive control: | C2C12 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate, NIH/3T3 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate, J774A.1 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:2,000-1:5,000 |
| Uniprot #: | SwissProt: Q14653 Human | P70671 Mouse Entrez Gene: 292892 Rat |
| Alternative names: | IIAE7 Interferon regulatory factor 3 IRF 3 IRF-3 IRF3 IRF3_HUMAN MGC94729 |
Images
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Fig1:
Western blot analysis of Phospho-IRF3 (S396) on different lysates with Rabbit anti-Phospho-IRF3 (S396) antibody (HA724666) at 1/5,000 dilution. Lane 1: C2C12 (Mouse myoblast) cell lysate Lane 2: C2C12 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: NIH/3T3 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 56 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724666, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47 kDa Observed band size: 47 kDa |
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Fig2:
Western blot analysis of Phospho-IRF3 (S396) on different lysates with Rabbit anti-Phospho-IRF3 (S396) antibody (HA724666) at 1/5,000 dilution. Lane 1: J774A.1 (Mouse monocyte macrophage) cell lysate Lane 2: J774A.1 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724666, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47 kDa Observed band size: 47 kDa |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724666, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724666, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724666, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.