Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [PSH27-06]
cat.: HA724667
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH27-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 108 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Desmoglein 3 aa 301-615. |
| Positive control: | A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, HaCaT (Human immortalized keratinocyte) cell lysate. |
| Subcellular location: | Cell membrane, Cell junction, desmosome, Cytoplasm, tight junction. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:500 1:200-1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P32926 Human |
| Alternative names: | 130 kD pemphigus vulgaris antigen 130 kDa pemphigus vulgaris antigen Balding Cadherin family member 6 CDHF 6 CDHF6 Desmoglein 3 Desmoglein 3 precursor Desmoglein-3 Desmoglein3 DKFZp686P23184 DSG 3 DSG3 DSG3_HUMAN Pemphigus vulgaris antigen PVA |
Images
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Fig1:
Western blot analysis of Desmoglein 3 on different lysates with Rabbit anti-Desmoglein 3 antibody (HA724667) at 1/5,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate Lane 3: HaCaT (Human immortalized keratinocyte) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724667, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 108 kDa Observed band size: 150 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: HaCaT (Human immortalized keratinocyte) / A549 (Human lung adenocarcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724667, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724667, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Esophagus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724667, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lung squamous cell carcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724667, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Application: Flow Cytometry
Species: Human Sample: A549 (Human lung adenocarcinoma cell) (Left) / HaCaT (Human immortalized keratinocyte) (Right) Blocking: 1% BSA + 10% normal goat serum , 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724667 (1/2,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.