Human/Rat/Monkey BDNF Recombinant Rabbit Monoclonal Antibody [PSH27-10]
cat.: HA724671
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Cynomolgus monkey |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH27-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BDNF aa 129-247 (HA211599). |
| Positive control: | Recombinant Human BDNF protein (HA211599). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH27-08] to Human/Rat/Monkey BDNF (Capture) (HA724669) and Recombinant Human BDNF protein (HA211599) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: P23560 Human | P23363 Rat |
| Alternative names: | Abrineurin ANON2 BDNF BDNF_HUMAN Brain Derived Neurotrophic Factor Brain-derived neurotrophic factor BULN2 MGC34632 Neurotrophin |
Images
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Fig1:
Sandwich ELISA analysis of Human BDNF matched pair antibodies Capture: HA724669, Human/Rat/Monkey BDNF Rabbit mAb [PSH27-08] Detector: HA724671, Human/Rat/Monkey BDNF Rabbit mAb [PSH27-10] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724669) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human BDNF protein (HA211599) starting from 1,000 pg/mL to 0 pg/mL and detect antibody (HA724671, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native BDNF in human, cynomolgus monkey and rat samples. Capture: HA724669, Human/Rat/Monkey BDNF Rabbit mAb [PSH27-08] Detector: HA724671, Human/Rat/Monkey BDNF Rabbit mAb [PSH27-10] Interpolated concentration of native BDNF was measured in duplicate at different sample concentrations.The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 11,819 pg/mL in human serum, 2,649 pg/mL in cynomolgus monkey serum and 2,462 pg/mL in rat serum. |
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Fig3:
Interpolated concentrations of spiked BDNF in cell culture media samples. Capture: HA724669, Human/Rat/Monkey BDNF Rabbit mAb [PSH27-08] Detector: HA724671, Human/Rat/Monkey BDNF Rabbit mAb [PSH27-10] The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.