BubR1 Recombinant Rabbit Monoclonal Antibody [PSH27-14]
cat.: HA724675
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH27-14 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 120 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BubR1 aa 1-200. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysates. |
| Subcellular location: | Nucleus, Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: O60566 Human Entrez Gene: 171576 Rat |
| Alternative names: | Beta homolg of S. cerevisiae BUB 1 Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles BUB 1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta Bub1A BUB1B BUB1B_HUMAN BUB1beta BUBR1 Budding Uninhibited by Benzimidazoles 1 beta Budding uninhibited by benzimidazoles 1 homolog beta (yeast) hBUBR1 MAD3/BUB1 related protein kinase MAD3/BUB1-related protein kinase MAD3L Mitotic checkpoint gene BUB1B Mitotic checkpoint kinase MAD3L Mitotic checkpoint serine/threonine protein kinase BUB1 beta Mitotic checkpoint serine/threonine-protein kinase BUB1 beta MVA1 OTTHUMP00000160319 Protein SSK1 SSK 1 SSK1 |
Images
|
Fig1:
Western blot analysis of BubR1 on different lysates with Rabbit anti-BubR1 antibody (HA724675) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 4: Ramos (Human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724675, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 120 kDa Observed band size: 120 kDa |
|
Fig2:
Western blot analysis of BubR1 on PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate with Rabbit anti-BubR1 antibody (HA724675) at 1/5,000 dilution.
Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724675, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 120 kDa Observed band size: 120 kDa |
|
Fig3:
Immunoprecipitation (IP) BubR1 was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724675 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724675 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA724675 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA724675 in HeLa cell lysate Exposure time: 3 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724675, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 120 kDa Observed band size: 120 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.