AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15]
cat.: HA724676
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH27-15 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AIM2 aa 1-200. |
| Positive control: | THP-1 treated with 200ng/mL hIFN-γ and 50ng/mL LPS for 24 hours add 300ng/mL BFA for last 20 hours cell lysate, HL-60 treated with 100ng/mL hIFN-γ for 24 hours cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, U266 (Human multiple myeloma cell) cell lysate. |
| Subcellular location: | Cytoplasm, Inflammasome, Nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O14862 Human |
| Alternative names: | Absent in melanoma 2 AIM 2 Aim2 AIM2_HUMAN Interferon-inducible protein AIM2 OTTHUMP00000035296 PYHIN4 |
Images
|
Fig1:
Western blot analysis of AIM2 on different lysates with Rabbit anti-AIM2 antibody (HA724676) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 2: THP-1 treated with 200ng/mL hIFN-γ and 50ng/mL LPS for 24 hours, add 300ng/mL BFA for last 20 hours cell lysate Lane 3: HL-60 (Human acute promyelocytic leukemia cell) cell lysate Lane 4: HL-60 treated with 100ng/mL hIFN-γ for 24 hours cell lysate Lane 5: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 6: U266 (Human multiple myeloma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724676, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39 kDa Observed band size: 39-43 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.