PIWIL2 Recombinant Rabbit Monoclonal Antibody [PSH27-16]
cat.: HA724677
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IP, mIHC |
| Clonality: | Monoclonal |
| Clone number: | PSH27-16 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse PIWIL2 aa 1-250. |
| Positive control: | Rat testis tissue lysate, Mouse testis tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IP mIHC |
1:2,000 1:250 1:500 1-2μg/sample 1:1,000 |
| Uniprot #: | SwissProt: Q8CDG1 Mouse Entrez Gene: 306011 Rat |
| Alternative names: | Piwi-like protein 2 EC:3.1.26. Piwil2 Mili |
Images
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Fig1:
mIHC analysis of mouse testis tissue (paraffin-embedded) with PIWIL2 (HA724677, 1/1,000) and Vimentin (ET1610-39, 1/10,000). The immunostaining was performed with the IRISKitCmTSA Kit (900802). DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Testis Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724677, 1/250, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724677, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724677, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of PIWIL2 protein in brain is consistent with the predicted expression pattern. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724677, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724677, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of PIWIL2 protein in brain is consistent with the predicted expression pattern. |
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Fig7:
Western blot analysis of PIWIL2 on different lysates with Rabbit anti-PIWIL2 antibody (HA724677) at 1/2,000 dilution.
Lane 1: Rat testis tissue lysate Lane 2: Rat brain tissue lysate Negative control: Brain (PMID: 16377660). Lysates/proteins at 30 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724677, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 109 kDa Observed band size: 109 kDa |
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Fig8:
Western blot analysis of PIWIL2 on different lysates with Rabbit anti-PIWIL2 antibody (HA724677) at 1/2,000 dilution.
Lane 1: Mouse testis tissue lysate Lane 2: Mouse brain tissue lysate Negative control: Brain (PMID: 16377660). Lysates/proteins at 30 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724677, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 109 kDa Observed band size: 109 kDa |
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Fig9:
Immunoprecipitation (IP)
PIWIL2 was immunoprecipitated in 0.2 mg mouse testis tissue lysate with HA724677 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724677 at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse testis tissue lysate (input) Lane 2: HA724677 IP in mouse testis tissue lysate Lane 3: Rabbit IgG instead of HA724677 in mouse testis tissue lysate Exposure time: 1 minute 50 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724677, 1/2,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 109 kDa Observed band size: 109 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.