c-Myb Recombinant Rabbit Monoclonal Antibody [PSH27-17]
cat.: HA724678
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH27-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human c-Myb aa 151-640. |
| Positive control: | RPMI8226 (Human multiple myeloma cell) cell lysate, SW620 (Human colon cancer cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, MOLT-4 (Human acute T-Cell leukemia cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, U-937 (Human acute monocytic leukemia cell) cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: P10242 Human |
| Alternative names: | C myb c myb protein (140 AA) c-myb protein (140 AA) c-myb_CDS c-myb10A_CDS c-myb13A_CDS c-myb14A_CDS c-myb8B_CDS Cmyb efg Myb MYB proto-oncogene transcription factor MYB_HUMAN ONCOGENE AMV Oncogene MYB Proto oncogene c Myb Proto-oncogene c-Myb Transcriptional activator Myb v myb avian myeloblastosis viral oncogene homolog v-myb avian myeloblastosis viral oncogene homolog |
Images
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Fig1:
Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA724678) at 1/5,000 dilution.
Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate Lane 2: SW620 (Human colon cancer cell) cell lysate Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Negative expression of c-Myb protein in A549 is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724678, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 72 kDa Observed band size: 72-80 kDa |
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Fig2:
Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA724678) at 1/5,000 dilution.
Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: MOLT-4 (Human acute T-Cell leukemia cell) cell lysate Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 4: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 5: U-937 (Human acute monocytic leukemia cell) cell lysate Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Negative expression of c-Myb protein in HeLa is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724678, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 72 kDa Observed band size: 72-80 kDa |
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Fig3:
Immunoprecipitation (IP)
c-Myb was immunoprecipitated in 0.2 mg Jurkat (Human T-lymphoblastic cells) cell lysate with HA724678 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724678 at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA724678 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA724678 in Jurkat cell lysate Exposure time: 20 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724678, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 72 kDa Observed band size: 72-80 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.