FOXF1 Recombinant Rabbit Monoclonal Antibody [PSH27-47]
cat.: HA724698
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH27-47 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, A375 (Human amelanotic melanoma cell) cell lysate, mouse lung tissue lysates. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200 |
| Uniprot #: | SwissProt: Q12946 Human | Q61080 Mouse Entrez Gene: 687536 Rat |
| Alternative names: | AI450827 FKH L5 FKHL 5 FKHL5 Forkhead (Drosophila) like 5 Forkhead box F1 Forkhead box protein F1 Forkhead drosophila homolog like 5 Forkhead like 5 Forkhead related activator 1 Forkhead related protein FKHL5 Forkhead related transcription factor 1 Forkhead-related activator 1 Forkhead-related protein FKHL5 Forkhead-related transcription factor 1 FOX F1 FOXF 1 foxf1 FOXF1_HUMAN FREAC 1 FREAC-1 FREAC1 HFH 8 MGC105125 |
Images
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Fig1:
Western blot analysis of FOXF1 on different lysates with Rabbit anti-FOXF1 antibody (HA724698) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: A375 (Human amelanotic melanoma cell) cell lysate A375 is a low expression (PMID: 29162563). Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724698, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 40 kDa |
|
Fig2:
Western blot analysis of FOXF1 on mouse lung tissue lysate with Rabbit anti-FOXF1 antibody (HA724698) at 1/5,000 dilution.
Lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724698, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 40 kDa |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724698, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724698, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of FOXF1 protein in heart is consistent with the predicted expression pattern. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724698, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724698, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of FOXF1 protein in heart is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.