Cathepsin S Recombinant Rabbit Monoclonal Antibody [PSH27-55]
cat.: HA724704
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH27-55 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cathepsin S aa 1-331. |
| Positive control: | THP-1 (Human acute monoblastic leukemia cell) cell lysate, THP-1 treated with 80nM TPA for 24 hours then treated with 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours cell lysate. |
| Subcellular location: | Lysosome, Secreted, Cytoplasmic vesicle, phagosome. |
| Recommended Dilutions:
WB |
1:4,000 |
| Uniprot #: | SwissProt: P25774 Human |
| Alternative names: | Cathepsin S CathepsinS CATS_HUMAN Ctss |
Images
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Fig1:
Western blot analysis of Cathepsin S on different lysates with Rabbit anti-Cathepsin S antibody (HA724704) at 1/4,000 dilution. Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 2: THP-1 treated with 80nM TPA for 24 hours then treated with 100ng/mL LPS for 7 hours , add 1μg/mL BFA for last 3 hours cell lysate Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Low expression of Cathepsin S protein in A549 cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724704, 1/4,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.