CD33 Recombinant Rabbit Monoclonal Antibody [PSH27-78]
cat.: HA724723
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH27-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD33 aa 1-282. |
| Positive control: | TF-1 (Human erythroleukemia cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, HL-60 (Human acute promyelocytic leukemia cell) cell lysate, HEL (Human erythroleukemia cell) cell lysate, RPMI8226 (Human multiple myeloma cell) cell lysate. |
| Subcellular location: | Cell membrane; Peroxisome. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500 |
| Uniprot #: | SwissProt: P20138 Human |
| Alternative names: | CD 33 CD33 CD33 antigen (gp67) CD33 antigen CD33 molecule CD33_HUMAN FLJ00391 gp67 My9 Myeloid cell surface antigen CD33 Myeloid cell surface antigen CD33 precursor Myeloid differentiation antigen CD33 p67 Sialic acid binding Ig like lectin 3 Sialic acid binding immunoglobulin like lectin 3 Sialic acid-binding Ig-like lectin 3 SIGLEC 3 Siglec-3 SIGLEC3 |
Images
|
Fig1:
Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA724723) at 1/2,000 dilution.
Lane 1: TF-1 (Human erythroleukemia cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 4: HL-60 (Human acute promyelocytic leukemia cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Negative Control: Jurkat (PMID: 30519686). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724723, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 67-75 kDa |
|
Fig2:
Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA724723) at 1/2,000 dilution.
Lane 1: HEL (Human erythroleukemia cell) cell lysate Lane 2: RPMI8226 (Human multiple myeloma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724723, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 67-75 kDa |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724723, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Placenta Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724723, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724723, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.