VE-Cadherin Recombinant Rabbit Monoclonal Antibody [PSH27-80]
cat.: HA724725
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: PSH27-80
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 0.5ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 88 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse VE-Cadherin aa 1-599.
Positive control: BEnd.3 (Mouse brain microvascular endothelial cell) cell lysate, Mouse lung tissue lysate, Mouse skeletal muscle tissue lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Mouse spleen tissue lysate, Rat lung tissue lysate, Rat kidney tissue lysate, Rat spleen tissue lysate.
Subcellular location: Cell junction, adherens junction, Cell membrane, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Tissue
  IHC-P
  IP
1:2,000
1:100
1:500
1-2μg/sample
Uniprot #: SwissProt: P55284 Mouse
Entrez Gene: 307618 Rat
Alternative names: 7B 4 7B4 7B4 antigen CADH5_HUMAN Cadherin 5 Cadherin 5 type 2 Cadherin 5, type 2 (vascular endothelium) Cadherin 5, type 2, VE cadherin (vascular epithelium) cadherin, vascular endothelial cadherin, vascular endothelial, 1 Cadherin-5 Cadherin5 CD 144 CD144 CD144 antigen CDH 5 CDH5 CDH5 protein Endothelial specific cadherin FLJ17376 OTTHUMP00000174777 Vascular endothelial cadherin Vascular epithelium cadherin VE Cad VE-cadherin VEC
Images
HA724725_1.jpg Fig1: Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (HA724725) at 1/2,000 dilution.

Lane 1: bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate (15 µg/Lane)
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate (15 µg/Lane)

Exposure time: 25 seconds; ECL: K1801

Low expression of VE-Cadherin protein in NIH/3T3 cell is consistent with the predicted expression pattern.

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724725, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 88 kDa
Observed band size: 90-150 kDa
HA724725_2.jpg Fig2: Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (HA724725) at 1/2,000 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lane 3: Mouse heart tissue lysate
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse spleen tissue lysate
Lane 6: Rat lung tissue lysate
Lane 7: Rat kidney tissue lysate
Lane 7: Rat spleen tissue lysate

Lysates/proteins at 30 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

The molecular weight observed is consistent with what has been described in the literature (PMID: 36631513).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724725, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 88 kDa
Observed band size: 90-150 kDa
HA724725_3.jpg Fig3: Application: Immunofluorescence (IF-tissue)

Species: Mouse
Tissue: Lung
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724725, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
HA724725_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Lung
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724725, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724725_5.jpg Fig5: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Lung
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724725, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724725_6.jpg Fig6: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: spleen
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724725, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724725_7.jpg Fig7: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: spleen
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724725, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724725_8.jpg Fig8: Immunoprecipitation (IP)

VE-Cadherin was immunoprecipitated in 0.2 mg bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate with HA724725 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724725 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: bEnd.3 cell lysate (input)
Lane 2: HA724725 IP in bEnd.3 cell lysate
Lane 3: Rabbit IgG instead of HA724725 in bEnd.3 cell lysate

Exposure time: 2 minutes 6 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature

Primary dilution: HA724725, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

Predicted band size: 88 kDa
Observed band size: 90-150 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.