Biotin Conjugated Human CEACAM6 Recombinant Rabbit Monoclonal Antibody [PSH27-95]
cat.: HA724737B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH27-95 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CEACAM6 aa 35-320 (HA211497). |
| Positive control: | Recombinant Human CEACAM6 protein (HA211497). |
| Subcellular location: | Cell membrane, Apical cell membrane, Cell surface. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH27-94] to Human CEACAM6 (Capture) (HA724736) and Recombinant Human CEACAM6 protein (HA211497) as the standard. The reference range value is 11.7-3,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P40199 Human |
| Alternative names: | Carcinoembryonic antigen related cell adhesion molecule 6 Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) Carcinoembryonic antigen-related cell adhesion molecule 6 CD 66c CD66c CD66c antigen CEA LIKE PROTEIN CEACAM 6 CEACAM6 CEAL CEAM6_HUMAN MGC93832 NCA Non specific cross reacting antigen Non-specific crossreacting antigen Normal cross reacting antigen Normal cross-reacting antigen |
Images
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Fig1:
Sandwich ELISA analysis of Human CEACAM6 protein matched pair antibodies Capture: HA724736, Human CEACAM6 Rabbit mAb [PSH27-94] Detector: HA724737, Human CEACAM6 Rabbit mAb [PSH27-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724736) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CEACAM6 protein (HA211497) starting from 6,000 pg/mL to 0 pg/mL and detect antibody (HA724737, Biotin, 0.05 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CEACAM6 in human samples. Capture: HA724736, Human CEACAM6 Rabbit mAb [PSH27-94] Detector: HA724737, Human CEACAM6 Rabbit mAb [PSH27-95] Interpolated concentration of native CEACAM6 was measured in duplicate at different sample concentrations and interpolated from the CEACAM6 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CEACAM6 concentration was determined to be 46,566 pg/mL in human serum. There was no detectable signal in A-172 cell supnatant. |
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Fig3:
Interpolated concentrations of native CEACAM6 in BxPC-3 Cell extract samples based on a 1,000 µg/mL extract load. Capture: HA724736, Human CEACAM6 Rabbit mAb [PSH27-94] Detector: HA724737, Human CEACAM6 Rabbit mAb [PSH27-95] Interpolated concentration of native CEACAM6 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CEACAM6 concentration was determined to be 149,767 pg/mL in BxPC-3 Cell extract. |
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Fig4:
Interpolated concentrations of spiked CEACAM6 in cell culture media samples. Capture: HA724736, Human CEACAM6 Rabbit mAb [PSH27-94] Detector: HA724737, Human CEACAM6 Rabbit mAb [PSH27-95] The concentrations of CEACAM6 were measured in duplicates, interpolated from the CEACAM6 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.